蛋白芯片詳細

信號通路磷酸化抗體芯片 > 更多 > ErbB信號通路磷酸化抗體芯片(PER239)

ErbB信號通路磷酸化抗體芯片(PER239)

       ErbB(Erythroblastic Leukemia Viral Oncogene Homolog)家族(erb1/EGFR, erb2/HER2, erb3/HER3 and erb4/HER4)屬于典型的跨膜RTKs(Receptor Tyrosine Kinases),在心臟、乳腺、中樞神經系統等器官的生長過程中均發揮重要作用。ErbB信號通路激活會引起一系列相關通路的同步激活,從而促進細胞增殖、遷移、粘附、分化乃至凋亡等生物現象的發生。 ErbB1和ErbB2與乳腺癌、卵巢癌、膀胱癌、前列腺癌、腦癌等諸多腫瘤的發生關系密切。在臨床腫瘤藥物研究中,ErbB已經成為目前最熱門的藥物靶點之一。


       ErbB信號通路磷酸化抗體芯片(PER239),采用三維高分子膜專利技術,在抗體芯片片基上共價結合239種高特異抗體,并運用特有的熒光標記技術進行樣本標記,以實現對ErbB經典信號通路的高覆蓋檢測??贵w芯片提供信號蛋白多個關鍵磷酸化位點的同步檢測,針對每一個特定蛋白磷酸化位點,設置一對抗體分別檢測其磷酸化(Phospho)和非磷酸化(non-Phospho)狀態。同時,抗體芯片可檢測多種已有文獻報道的非ErbB經典通路的信號蛋白,大大擴展ErbB單一信號通路研究的延伸性。


抗體芯片特點:


1. 芯片規格為76 x 25 x 1 mm;                

2. 實現單一信號通路全面篩選;                 

3. 每種抗體設置6次技術重復;                 

4. 適用于組織、細胞等多類型樣本;                

5. 5x106細胞、200μg總蛋白量即可滿足實驗;              

6. 每個檢測位點設有磷酸化和非磷酸化配對抗體;                

7. 可通用于人、小鼠、大鼠等多類型模式生物檢測。                

                                        

抗體芯片原理:                

 


   

                                        

                               

抗體芯片列表:


  動態箭頭.gif詳細列表下載


抗體芯片文獻:  
 

1.      Liu D, et al. C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. Nat Commun. 2018, 9(1): 1739. (華中科技大學同濟醫院)
2.      Lei miao 
Yin, et al. 
Transgelin-2 as a therapeutic target for asthmatic pulmonary resistanceSci Transl Med,2018, 10(427).(上海中醫藥大學)
3.      Venkatesh, Humsa S., et al. Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.Nature, 2017, 549(7673): 533-537. 
4.      Gao Y, et al. Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis.Natstruct Mol Biol, 2016, 23(5): 441-449. (中國科學院生物物理研究所)

5.     Jiang HL et al. SSRP1 uppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde Signaling, Cancer Res, 2016, 76(4): 952-64.(復旦大學附屬腫瘤醫院)

6.      Kuang XY et al. The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasis, Cancer Lett, 2016, 377(1): 87-96.復旦大學附屬腫瘤醫院)

7.      Chen Y, et al. The hepatitis B virus X protein promotes pancreatic cancer through modulation of the PI3K/AKT signaling pathwayCancer Lett, 2016, 380(1): 98-105. (浙大第二附屬醫院)

8.       Zhu Y et al. Long non-coding RNA LOC572558 inhibits bladder cancer cell proliferation and tumor growth by regulating the AKT-MDM2-p53 signaling axisCancer Lett, 2016, 04(3).(復旦大學附屬腫瘤醫院)

9.        Luo L L, Zhao L, Wang Y X, et al. Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5: 17336.  (中山大學附屬腫瘤醫院)

10.       Zhu Y P, Wan F N, Shen Y J, et al.  Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.(復旦大學附屬腫瘤醫院)
11.      Wang T, Han S, Wu Z, et al.  XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer. Biochem Bioph Res Co, 2015, 464(2): 635-641. (上海長征醫院)

12.      Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt-p27(Kip1) axis. Mol Carcinogen, 2015, 54(12): 1678-85. (福建醫科大學附屬協和醫院)

13.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (復旦大學附屬腫瘤醫院)

14.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater, 2016, 29: 320-32.  (同濟大學)

15.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015, 55(11): 1739-1746. (河北大學)

16.      Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis,2014, 35(7): 1573-1581.(上海交通大學醫學院)

17.      Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res, 2014, (24): 204-217. (上海仁濟醫院) 

18.      Xu N et al. Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-kB and JAK-STAT kinase signaling pathways. Int J Mol Med, 2013, 32(3): 729-735.(吉林大學)

19.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013, 8(4): e61195.(第三軍醫大學)

20.      Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen. 2012, 20(5): 778-785.

21.       Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death Dis, 2012, 3: e406.(西安交通大學)