蛋白芯片詳細

Luminex液相芯片 > Rat > Luminex 大鼠TGF-β因子 液相懸浮芯片

Luminex 大鼠TGF-β因子 液相懸浮芯片

 


        Rat  TGF-beta  液相懸浮芯片
是在Luminex公司授權的xMAP技術基礎上發展起來的基于微孔板的多重檢測抗體芯片技術。Luminex的xMAP技術是將高度特異的捕獲單克隆抗體偶聯到不同熒光標記的磁珠上,將不同種類磁珠混合后懸浮于96孔微孔板中。進一步,加入生物素標記的高親和配對二抗結合SA-PE進行信號放大,以實現對多種微量細胞因子的有效檢測。利用梯度稀釋的標準品檢測信號構建標準曲線,實現大量目標樣本中多因子的同時檢測和準確定量。

               

 液相芯片特點:


  1. 每種抗體設置50次技術重復;

  2. 可批量檢測80個樣本中的多重因子;

  3. 可根據研究要求定制個性化檢測芯片;

  4. 雙抗夾心&熒光檢測法有效檢測微量指標,下限達pg/mL;

  5. 50μL的液質樣本、200μg的總蛋白量即可完成多指標檢測;

  6. 適用于血清/漿、培養上清及細胞、組織裂解液等多種生物樣本;

  7. 可以有效避免采用傳統方法所引入的批次間實驗誤差;

  8. 可大限度擴大單個樣本的信息量并可做不同分析物間的交互分析。

 

液相芯片原理:




液相芯片列表:

 

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詳細列表下載

 
液相芯片文獻:


  1. Zhu X, Zhang K, He W, et al. Proinflammatory status in the aqueous humor of high myopic cataract eyes. Exp Eye Res, 2016, 142: 13-18.  (復旦大學附屬眼耳鼻喉醫院

  2. Wei Y, Liu B, Sun J, et al. Regulation of Th17/Treg function contributes to the attenuation of chronic airway inflammation by icariin in ovalbumin-induced murine asthma model.Immunobiology, 2015. (上海華山醫院

  3. Liu Q, et al. Targeting the surface protein SasX by active and passive vaccination to reduce Staphylococcus aureus colonization and infection. Infect Immun, 2015: IAI. 02951-14. (上海交通大學醫學院

  4. Liu Y H, et al. Omega-3 Fatty Acid Intervention Suppresses Lipopolysaccharide-Induced Inflammation and Weight Loss in Mice. Mar Drugs, 2015, 13(2): 1026-1036. (北京301醫院

  5. Fan M, Zhang J, Wang Z, et al. Phosphorylated VEGFR2 and hypertension: potential biomarkers to indicate VEGF-dependency of advanced breast cancer in anti-angiogenic therapy. Breast Cancer Res Tr, 2014, 143(1): 141-151.  (復旦大學

  6. Ye S, Li Z L, Luo D, et al. Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma. Oncotarget, 2014, 5(14): 5439.  (中山大學癌癥研究中心

  7. Xu C,Ju X,Song D, et al. An association analysis between psychophysicalcharacteristics and genome-wide gene expression changes in human adaptation to the extreme climate at the Antarctic Dome Argus. Mol Psychiatr, 2014. (北京醫科院

  8. Chen X, Gan Y, Li W, et al. The interaction between mesenchymal stem cells and steroids during inflammation. Cell Death Dis, 2014, 5(1): e1009.  (上海交通大學

  9. Ye S, et al. Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma. Oncotarget, 2014, 5(14): 5439. (中山大學

  10. Xie Y, et al. Effect of Intestinal Microbiota Alteration on Hepatic Damage in Rats with Acute Rejection After Liver Transplantation. Microbial Ecol , 2014, 68(4): 871-880. (浙江大學醫學院

  11. Liu B, et al. Severe influenza A(H1N1)pdm09 infection induces thymic atrophy through activating innate CD8(+)CD44(hi) T cells by upregulating IFN-γ. Cell Death Dis, 2014, 5(10): e1440. (首都醫科大學

  12. Gao R, et al. Cytokine and chemokine profiles in lung tissues from fatal cases of 2009 pandemic influenza A (H1N1): role of the host immune response in pathogenesis. Am J Pathol , 2013, 183(4): 1258-1268. (中國疾控中心

  13. You S, et al. Inhibitory effects and molecular mechanisms of garlic organosulfur compounds on the production of inflammatory mediators. Mol Nutr Food Res , 2013, 57(11): 2049-2060.

  14. Zhao J, et al. Spinal interleukin-33 and its receptor ST2 contribute to bone cancer-induced pain in mice. Neuroscience, 2013, 253: 172-182. (湖南農業大學

  15. Wakao H, Yoshikiyo K, Koshimizu U, et al. Expansion of functional human mucosal-associated invariant T cells via reprogramming to pluripotency and redifferentiation. Cell Stem Cell, 2013, 12(5): 546-558.

  16. Zhao J, Zhang H, Liu S B, et al. Spinal interleukin-33 and its receptor ST2 contribute to bone cancer-induced pain in mice. Neuroscience, 2013, 253: 172-182. (復旦醫學院

  17. You S, Nakanishi E, Kuwata H, et al. Inhibitory effects and molecular mechanisms of garlic organosulfur compounds on the production of inflammatory mediators. Mol Nutr Food Res, 2013, 57(11): 2049-2060. (湖南農業大學

  18. Li C, Yang P, Sun Y, et al. IL-17 response mediates acute lung injury induced by the 2009 pandemic influenza A (H1N1) virus. Cell Res, 2012, 22(3): 528-538. (北京協和醫學院

  19. Zhang X, Zhang Y, Tao B, et al. Loss of Shp2 in alveoli epithelia induces deregulated surfactant homeostasis, resulting in spontaneous pulmonary fibrosis. FASEB J, 2012, 26(6): 2338-2350. (浙大醫學院